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The captured troponin is then reacted by using a second antibody and, in some assays, a 3rd antibody coupled to an indicator molecule.[36] The assays vary from each other by the categories of antibody applied, by the epitopes to which they bind, and by the sort of indicator molecule that may be applied.[46]

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Following adding streptavidin-coated microparticles, the advanced is certain to the stable stage via conversation with biotin and streptavidin. The response combination is aspirated into the measuring mobile, exactly where the microparticles topanwin rtp are magnetically captured onto the area of your electrode. Unbound substances are eradicated, and a voltage is placed on the electrode.[31]

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